| Article number | Wells/Plate | Plate frame colour | Bottom | Bottom thickness | Plates/bag | Plates/Sales Unit | Lid | Sterile |
|---|---|---|---|---|---|---|---|---|
| 5160-30 | 1 | clear | Cover glass | 145µm (1.0) | 2/bag | 30/box | + | + |
| 5160-150 | 1 | clear | Cover glass | 145µm (1.0) | 2/bag | 150/box | + | + |
| 6160-30 | 1 | clear | Cover glass | 170µm (1.5) | 2/bag | 30/box | + | + |
| 6160-150 | 1 | clear | Cover glass | 170µm (1.5) | 2/bag | 150/box | + | + |
| 6170-30 | 1 | clear | Cover glass | 170µm (1.5H) high-precision glass | 2/bag | 30/box | + | + |
| 6170-150 | 1 | clear | Cover glass | 170µm (1.5H) high-precision glass | 2/bag | 150/box | + | + |
Life Cell Imaging, Fluorescence-Correlation Spectroscopy (FCS), Confocal Laser Scanning Microscopy (LSM), Total Internal Reflection Fluorescence (TIRF), Differential Interference Contrast (DIC/Nomarski), Fluorescence Resonance Energy Transfer (FRET), Fluorescence Recovery after Photobleaching (FRAP), Weakly fluorescent stains / proteins, Fluorescence In-Situ Hybridization (FISH), Immune histology, Low intensity fluorescence, Microinjection/Micromanipulation, Cytometry, (direct) Stochastic Optical Reconstruction Microscopy ((d)STORM)
The product Imaging Dish is our entry solution for high resolution microscopy of living and fixed cells. The bottom of the 35 mm petri dish is made of cover glass. The central cover glass area has a diameter of 18 mm. The level of the cover glass is 2 mm lower than the lower edge level of the surrounding polystyrene base body. Due to this feature it is possible to concentrate the cells during the seeding on the glass surface.
Several features support a user friendly manipulation of the dish. The grip zone of the base body is increased and allows a safe picking of the dish. The lid contains spacers for a good ventilation. The lid can be easily removed for cell treatment or examination. The dishes are delivered sterile in bags of 2 dishes.
The focus level of the glass area is placed only 400 µm above the basement line of the base body. High quality cover glasses made of borosilicate glass are used as glass bottom. Variants with cover glass thickness 1.0 (145 µm ± 15 µm), 1.5 (175 µm ± 15 µm) and 1.5H (high-precision glass of 170 µm ± 5µm) are offered. The glass bottom have an excellent planarity, focus control and superior imaging quality.
The product Imaging Dish is now also available with a “high-precision glass”. These are ideal for high-resolution analyses, e.g. STED or (d)STORM.
The macroscopic orientation of the dishes is supported by four marks (N-O-S-W). The twelve corner grip zone of the dish body enables an easy orientation and positioning in combination with our Imaging Dish Stage Frame. Therefore the same orientation of the dish can be established even if the dish had to be removed from the microscope stage in between two observation sessions.
They are free of detectable DNase, RNase and human DNA, free of detectable endotoxins and free of cytotoxic substances.
TC = Tissue Culture, amine coated surface, suitable for most adherend cell lines (e.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (e.g. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.
