Imaging Plate CG

Multiwell plates with cover glass bottom (145 or 170 µm thickness)

Information

Article numberWells/PlatePlate frame colourBottomBottom thicknessPlates/bagPlates/Sales UnitLidSterile
5221-206blackCover glass145 µm (1.0)1/bag20/box++
5231-2024blackCover glass145 µm (1.0)1/bag20/box++
5241-2096blackCover glass145 µm (1.0)1/bag20/box++
5222-206blackCover glass170 µm (1.5)1/bag20/box++
5232-2024blackCover glass170 µm (1.5)1/bag20/box++
5242-2096blackCover glass170 µm (1.5)1/bag20/box++
  • Low skirt format, viewing areas of imaging plates accessible for immersion objectives
  • Tissue culture surface
  • Excellent planarity

Life cell imaging, immunohistochemistry, super high resolution microscopy, fluorescence correlation spectroscopy (FCS), laser scanning microscopy, high content analysis (HCA)

Imaging Plates CG are black multiwell plates compliant to the SBS (Society for Biomolecular Screening) standard-format with cover glass bottoms made from borosilicate glass. The plate bodies are made from polystyrene and tightly bonded to the cover glass. Our Multiwellplates can be used for high resolution Live Cell Imaging. In addition direct fixation and staining within the plates are possible (formaldehyde, glutaraldehyde, alcohols and acetone can be used). Acetone should not be applied more than 10 minutes because the plate body is made from polystyrene and subsequent to the material reaction the cover glass may detach. Imaging Plates CG can be used at temperatures between -20°C and 50°C. Due to the cover glass bottom the application of high resolution immersion objectives with high numerical aperture is possible. Water, oil and glycerin can be used as immersion media.

Cover glasses are still the best choice as object carrier for high resolution light microscopy. Imaging Plate CG products are equipped with a bottom made from 145µm or 170 µm thick borosilicate glass. Cell adhesion, spreading and distribution are improved by our proven plasma surface modification technology. To support this, the glass is modified by plasma treatment to increase the number of amino groups on the substrate surface.

All Imaging Plates have a black plate body to suppress cross signals and to prevent excitation of regions out of interest in epifluorescence applications. Each single plate has a lid, is individually packaged, and is distributed as a unit box of 20 pieces. The lid contains condensation rings, stacking pins and spacers for an optimal ventilation

They are free of detectable DNase, RNase and human DNA, free of detectable endotoxins and free of cytotoxic substances.

Note: All polymer plate bodies react to temperature shifts with dimensional changes. Please take care that the plate and its environment have equal temperature during microscopy to minimize focal shifts and reduce refocusing.

Specification

Article number
5221 / 5222
Format
6 Well
Article number
5231 / 5232
Format
24 Well
Article number
5241 / 5242
Format
96 Well
Material
Polystyrene
Bottom
Borosilicate, hydrolytic class 1
Thickness
1.0, 145 µm ±10% / 1.5, 170 µm ±10%
Refractive Index
1,52
Abbe’s number
50
Light transmission @ 310 nm
> 85%
Light transmission @ 360 nm
> 90%
Surface quality
Tissue culture surface
Lid
Yes
Sterile
Yes

TC = Tissue Culture, amine coated surface, suitable for most adherend cell lines (e.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (e.g. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

Technical Data

Article number
5221 / 5222
5231 / 5232
5241 / 5242
Outer Plate diameter (LxWxH)
5221 / 5222: 127,76 x 85,48 x 15,00 mm
5231 / 5232: 127,76 x 85,48 x 15,00 mm
5241 / 5242: 127,76 x 85,48 x 14,35 mm
Depth per well
5221 / 5222: 14,60 mm
5231 / 5232: 14,60 mm
5241 / 5242: 13,95 mm
Well Diameter (focal plane)
5221 / 5222: 32,00 mm
5231 / 5232: 13,20 mm
5241 / 5242: 6,00 mm
Distance well center to well center
5221 / 5222: 36,00 mm
5231 / 5232: 18,00 mm
5241 / 5242: 9,00 mm
Distance from center of well A1 to longitudinal plate border
5221 / 5222: 24,74 mm
5231 / 5232: 15,74 mm
5241 / 5242: 11,24 mm
Distance from center of well A1 to short plate border
5221 / 5222: 27,88 mm
5231 / 5232: 18,88 mm
5241 / 5242: 14,38 mm
Distance lower plate level (skirt rim) to focal plane
400 µm
Planarity per well
< 10 µm
Planarity per plate
< 50 µm
Total volume / well
5221 / 5222: 12,112 mL
5231 / 5232: 1,880 mL
5241 / 5242: 0,428 mL
Suggested working volume
5221 / 5222: 2000-4000 µL
5231 / 5232: 500-1000 μL
5241 / 5242: 100-200 µL
Article number
5221 / 5222
5231 / 5232
5241 / 5242
Weight 20-piece sales unit
5221 / 5222: 1,5 kg
5231 / 5232: 1,8 kg
5241 / 5242: 2,0 kg
Box Dimension 20-piece sales unit
315 x 240 x 155 mm
Quantity/Box
20
Quantity/Inner Pack
1
Sales Units/Shipping package
A Shipping package includes minimum 1 to maximum 5 Sales Units
Other
Larger order amount on request
Flyer
Drawing Imaging Plate (6 Well)
Drawing Imaging Plate (24 Well)
Drawing Imaging Plate (96 Well)
Drawing Lid (24 Well)
Drawing Lid (96 Well)

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