| Article number | Wells/Plate | Plate frame colour | Bottom | Bottom thickness | Plates/bag | Plates/Sales Unit | Lid | Sterile |
|---|---|---|---|---|---|---|---|---|
| 3221-20 | 6 | black | Film | 25 µm | 1/bag | 20/box | + | + |
| 3231-20 | 24 | black | Film | 25 µm | 1/bag | 20/box | + | + |
| 3241-20 | 96 | black | Film | 25 µm | 1/bag | 20/box | + | + |
Life cell imaging, Laser Scanning Confocal Microscopy (LSM), Fluorescence Resonance Energy Transfer (FRET), Fluorescence Recovery After Photobleaching (FRAP), Low intensity fluorescence, Immunohistology, High Content Analysis/Screening (HCA/HCS), Phototoxicity studies, Hypoxia/Hyperoxia studies, Metabolic highly active cells
Imaging Plates FC are black multiwell plates compliant to the SBS (Society for Biomolecular Screening) standard format and have a 25 µm thick film base made of a fluoropolymer (FC = fluorocarbon film). During the manufacturing the thin film is stretched around each individual well bottom to provide tension and planarity. Because of the film bottom characteristics the plates show excellent light transmission rates even for UV-A and UV-B light. The intrinsic fluorescence of the material is much lower if compared to conventional polystyrene bottoms with significant reduction of background signals. Furthermore the film bottom enables a high gas transfer through. Oxygen supply and equilibration with the atmosphere are achieved directly through the plate bottom.
The plate bodies are made from polystyrene and tightly bonded to the FC film. Imaging Plates FC can be used for high resolution Live Cell Imaging. In addition direct fixation and staining within the plates is possible (formaldehyde, glutaraldehyde, alcohols and acetone can be used). Acetone should not be applied more that 10 minutes because the plate body is made from polystyrene and subsequent to the material reaction the film bottom may detach. Imaging Plates FC can be used between -20°C and 50°C. Due to the thin film bottom the application of high resolution immersion objectives with high numerical aperture is possible. For optimal focus control when objectives with 40x magnification and higher the use of objectives with correction rings or collars is recommended. Water, oil and glycerin can be used as immersion media.
40-fachen Vergrößerung und höher wird die Verwendung von Objektiven mit Korrekturringen oder -manschetten empfohlen. Wasser, Glycerin oder Öl können als Immersionsflüssigkeit verwendet werden.
They are free of detectable DNase, RNase and human DNA, free of detectable endotoxins and free of cytotoxic substances.
Note: All polymer-based plate bodies react to temperature shifts with dimensional changes. Please take care that the plate and its environment have equal temperature during microscopy to minimize focal shifts and reduce refocusing.
TC = Tissue Culture, amine coated surface, suitable for most adherend cell lines (e.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (e.g. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.
