Imaging Chamber Cover Glass

Chambered cover glass with removable chambers

Information

Article numberWells/PlatePlate frame colourBottomBottom thicknessPlates/bagPlates/Sales UnitLidSterile
8001-161clearCover glass170 µm (1.5)1/bag16/box++
8001-801clearCover glass170 µm (1.5)1/bag80/box++
8002-161clearCover glass170 µm (1.5)1/bag16/box++
8002-801clearCover glass170 µm (1.5)1/bag80/box++
8004-161clearCover glass170 µm (1.5) 1/bag 16/box++
8004-801clearCover glass170 µm (1.5)1/bag80/box++
8008-161clearCover glass170 µm (1.5)1/bag16/box++
8008-801clearCover glass170 µm (1.5)1/bag80/box++
  • Tool free removable chambers
  • Tissue culture surface
  • High temperature and chemical resistance

Life cell imaging, immunohistochemistry, fluorescence in-situ hybridization, super high resolution microscopy, fluorescence correlation spectroscopy (FCS), laser scanning microscopy

Imaging Chamber products are cell culture vessels with a removable chamber body and cover glass bases with a glass thickness of 170 µm. Chambers are made of high- performance polymers and are sealed against the glass bottoms by biological inert silicone adhesive. At the end of the experiments the glass can be detached from the chamber and used for further processing or archiving.

The high performance polymer of chamber bodies and lids withstands temperatures up to 90°C. Due to this temperature stability the Imaging Chambers show also excellent stability of the focus plane even though the chambers are exposed to temperature shifts (e.g. when the chambers are transported from the incubator to the microscope). Furthermore the Imaging Chambers are resistant against many chemicals (e.g. acetone, DMSO, alcohols). Therefore the full products are suitable for in-situ hybridization technologies as well as for a wide range of fixation and staining protocols.

The cover glasses of the Imaging Chambers CG are made of borosilicate glass compliant to hydrolytic class I with a thickness of 170 µm. Imaging Chambers CG with cover glass bottoms can be used for high resolution microscopy with immersion objectives (with high numerical aperture).

Cell adhesion, spreading and distribution are supported by over improved surface modification. Imaging Chambers are available in 1, 2, 4 and 8 well format.

They are free of detectable DNase, RNase and human DNA, free of detectable endotoxins and free of cytotoxic substances.

Specification

Article number
8001
Format
1 Well
Article number
8002
Format
2 Well
Article number
8004
Format
4 Well
Article number
8008
Format
8 Well
Material
COC
Bottom
Borosilicate, hydrolytic class 1
Thickness
170 µm
Refractive Index
1,52
Abbe’s number
50
Light transmission
> 90%
Surface quality
Tissue culture surface
Lid
Yes
Sterile
Yes

TC = Tissue Culture, amine coated surface, suitable for most adherend cell lines (e.g. HEK U293, HepG2, MCF-7). Sensitive cell lines (e.g. PC-12) and some primary cell types benefit from additional surface coatings with ECM proteins or equivalent coatings.

Technical Data

Article number
8001
Article diameter (LxWxH)
58 x 26 x 10 mm
Outer chamber diameter (LxWxH)
58 x 26 x 10 mm
Imaging area / well
10,84 cm²
Total volume / well
11 mL
Suggested working volume
2000 µL
Article number
8002
Article diameter (LxWxH)
58 x 26 x 10 mm
Outer chamber diameter (LxWxH)
58 x 26 x 10 mm
Imaging area / well
5,04 cm²
Total volume / well
5,5 mL
Suggested working volume
1000 µL
Article number
8004
Article diameter (LxWxH)
58 x 26 x 10 mm
Outer chamber diameter (LxWxH)
58 x 26 x 10 mm
Imaging area / well
2,14 cm²
Total volume / well
2,2 mL
Suggested working volume
500 µL
Article number
8008
Article diameter (LxWxH)
58 x 26 x 10 mm
Outer chamber diameter (LxWxH)
58 x 26 x 10 mm
Imaging area / well
0,88 cm²
Total volume / well
0,9 mL
Suggested working volume
300 µL
Article number
8001, 8002, 8004, 8008
Weight 16-piece sales unit
0,3 kg
Weight 80-piece sales unit
1,8 kg
Box Dimension 16-piece sales unit
135 x 143 x 90 mm
Box Dimension 80-piece sales unit
315 x 240 x 155 mm
Quantity/Box
16 / 80
Quantity/Inner Pack
1
Sales Units/Shipping package
A Shipping package includes minimum 1 of 16-piece sales unit to maximum 5 of 80-piece sales unit
Other
Larger order amount on request
Flyer
Flyer handling
Drawing 8001
Drawing 8002
Drawing 8004
Drawing 8008

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